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KMID : 0861020220370030021
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Korea Journal of Herbology
2022 Volume.37 No. 3 p.21 ~ p.27
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Single Oral Dose Toxicity Test of Lythri Herba Water Extracts in Sprague-Dawley Rat
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Kim Hee-Young
Park Yea-Jin
Shin Su
Choi Min-Woo
Hong Eun-Jin
Shin Young-Woo
Cha Yun-Yeop
An Hyo-Jin
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Abstract
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Objectives : Single oral dose toxicity test of Lythri Herba water extracts (LHWE) in Sprague-Dawley (SD) rat was
performed to determine approximate lethal dose (ALD) of LHWE.
Methods : This test was progressed according to OECD Guidelines for the Testing of Chemicals : acute oral toxicity.
After adaptation of 7 days, SD rats were divided into 2 groups : vehicle control and 5000 mg/kg LHWE-treated group.
Each group consisted of 5 female rats and 5 male rats. Vehicle or 5000 mg/kg LHWE was orally administrated once a day. Survival rates, general toxicity, and changes of body weight were investigated for 14 days after administration.
On the last day of examination, the weight of all animals was measured and an autopsy was performed. All internal organ abnormalities were checked macroscopically and their findings were recorded.
Results : In both groups, dead animals were not observed. During 14 days of administration, abnormal clinical signs were not detected. There was also no significant difference in weight gains between each group. Autopsy analysis showed that one case of the LHWE-treated female group had retention of clear fluid in the uterus; however, it was not considered to be affected by LHWE administration. Moreover, abnormal findings were not discovered in the control male group and the LHWE-treated male group.
Conclusions : These results suggest that the ALD of LHWE exceed 5000 mg/kg and single oral administration of LHWE below 5000 mg/kg is nontoxic.
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KEYWORD
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Lythri Herba, approximate lethal dose, acute oral toxicity, Sprague-Dawley rat
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